Two infectious forms of bacteriophage phi X 174
نویسندگان
چکیده
منابع مشابه
Gene D of bacteriophage phi X 174: absence of transcriptional and translational regulatory properties.
The only evidence that a gene product of phi chi 174 is involved in the regulation of phage transcription was presented by Clements and Sinsheimer (1974) for gene D. I. found, in attempting to investigate this possibility further, that the D protein had no regulatory effect. When the D protein was eliminated by various nonsense mutations, all other phage proteins were synthesized at rates nearl...
متن کاملGenetic Map of Bacteriophage 4 X 174
Bacteriophage 46X174 temperature-sensitive and nonsense mutations in eight cistrons were mapped by using two-, three-, and four-factor genetic crosses. The genetic map is circular with a total length of 24 X 104 wt recombinants per progeny phage. The cistron order is D-E-F-G-H-A-B-C. High negative interference is seen, consistent with a small closed circular deoxyribonucleic acid molecule as a ...
متن کاملDNA packaging intermediates of bacteriophage X 174
Background: Like many viruses, bacteriophage X174 packages its I)NA genome into a procapsid that is assembled from structural intermediates and scaffolding proteins. The procapsid contains the structural proteins F, G and H, as well as the scaffolding proteins B and D. Provirions are formed by packaging of DNA together with the small internal J proteins, while losing at least some of the B scaf...
متن کاملNanopore Sequencing of the phi X 174 genome
Nanopore sequencing of DNA is a single-molecule technique that may achieve long reads, low cost, and high speed with minimal sample preparation and instrumentation. Here, we build on recent progress with respect to nanopore resolution and DNA control to interpret the procession of ion current levels observed during the translocation of DNA through the pore MspA. As approximately four nucleotide...
متن کاملGenetic information analysis of bacteriophage phiX 174.
The genetic information of phix 174 genome (genes and intermediate segments) is analyzed in terms of its independent (D1 index) and dependent information (D2 and D3 Markovian indexes), as well as of its ability to generate secondary structure. Genes B and E, enclosed in A and D respectively, have: 1) values of D1 and D3 indexes closer to the theoretical random distribution curves than those of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Virology
سال: 1977
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.23.2.439-442.1977